Oxidative-Stress and Atherosclerosis Laboratory

 

  • Description
    • The laboratory's interests lie with elucidating the mechanisms underlying the accelerated development of atherosclerosis during aging and, more particularly, with the role of HDL (high-density lipoproteins or "good" cholesterol) and the parameters regulating their antiatherogenic properties. More specifically, its research lies with the mechanisms by which paraoxonase (PON1), a protein associated exclusively with HDL, regulates the anti-inflammatory and antioxidant properties of HDL and its ability to mediate reverse cholesterol transport.

      Another of the laboratory's themes deals with preventing cardiovascular diseases (CVDs). The prevention targeted hinges on nutritional interventions (olive oil, lipid-lowering agents, and/or antioxidants), on one hand, and on developing methods that could be used clinically in identifying atherosclerotic plaque, on the other. In conjunction with its collaborators, the laboratory recently developed a technique for locating and quantifying arterial inflammation based on positron emission tomography (PET). Moreover, the laboratory wants to use PET and CT imaging to identify the most specific serum biomarkers for arterial inflammation and the fragility of atherosclerotic plaque.

      In addition, the laboratory is striving to elucidate the role of HDL and PON1 in Alzheimer's disease. 
  • Expertise
      • Cell culture
      • Animal-model study
      • Clinical study
      • Analysis using high-performance liquid chromatography (HPLC)
      • Molecular-biology techniques to analyze DNA and proteins (equipment: Li-COR Odyssey FC, Thermo Fisher NanoDrop 2000 V, PerkinElmer Victor X5)
      • Quantitative PCR genetic analysis (device: Stratagene multiplex quantitative PCR)
      • Enzyme assays
      • Flow cytometry
      • Size and density analysis of VLDL, LDL, and HDL lipoproteins using the gel-tube method (Quantimetrix Lipoprint)

      Collaboration

      • Positron-emission-tomography (PET) imaging with [18F]-fluorodeoxyglucose (18-FDG)
      • Computed tomography (CT) 
  • Researcher
    • Abdel Khalil

 

Coordinator
Perreault, Audrey
819 780-2220 , 45249



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